Journal: Acta Pharmaceutica Sinica. B

Article Title: A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein

doi: 10.1016/j.apsb.2020.02.009

Figure Lengend Snippet: Possible role of NF- κ B and NF- κ B-mediated pro-survival signals in TP/LPS-induced hepatotoxicity. (A) Experimental design to detect the time-dependent changes of NF- κ B and its related pro-survival factors. (B)–(G) Relative mRNA levels of NF- κ B target genes, including Ciap1 , Ciap2 , Flip L , Xiap , Tnfaip3 , and Nfkbia , were detected by qPCR with tubulin as the internal control ( n = 6). (H)–(L) Representative Western blots and relative intensity of protein bands of FLIP L , CIAP1, XIAP, and I κ B- α after the treatment of TP and LPS with tubulin as the loading control ( n = 4–6). (M) Representative photomicrographs of liver sections by IHC for P65 1 h after LPS application (200 × ). Scale bar = 50 μm. Results were expressed as mean ± SEM and statistical analysis was performed using Two-way ANOVA following by Tukey's multiple comparison test. *, # , $ P <0.05, **, ## , $$ P <0.01, ***, ### , $$$ P <0.001; ns, no statistical difference.

Article Snippet: Antibody against RIPK1 (17519-1-AP), mixed lineage kinase domain like pseudokinase (MLKL, 66675-1-Ig), myeloperoxidase (MPO, 22225-1-AP), and CIAP1 (10022-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques: Control, Western Blot, Comparison

Journal: Acta Pharmaceutica Sinica. B

Article Title: A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein

doi: 10.1016/j.apsb.2020.02.009

Figure Lengend Snippet: The primer sequences used for qPCR assay in mice.

Article Snippet: Antibody against RIPK1 (17519-1-AP), mixed lineage kinase domain like pseudokinase (MLKL, 66675-1-Ig), myeloperoxidase (MPO, 22225-1-AP), and CIAP1 (10022-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques:

Journal: Leukemia

Article Title: Smac-mediated sensitization of human B-lymphoma cells to staurosporine- and lactacystin-triggered apoptosis is apoptosome-dependent.

doi: 10.1038/sj.leu.2404660

Figure Lengend Snippet: Figure 1 Resistance to STS-induced apoptosis and elevated expression of cIAP2 in human B-lymphoma cells. (a) BL-derived cell lines were treated with STS (1 mM) for 12 h and apoptosis was determined by fluorescence microscopic evaluation of Hoechst 33342-labeled cells. Data shown are mean7s.d. (n ¼ 3). (b) Total cellular extracts (30 mg) obtained from BL-derived cell lines were subjected to immunoblotting using antibodies against cIAP1, cIAP2 and XIAP. Protein lysates from the apoptosis-sensitive Jurkat leukemia cell line were included for comparison.

Article Snippet: Antibodies to the following proteins were used: b-actin (Sigma), Apaf-1 (Chemicon, Temecula, CA, USA), cIAP1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), cIAP2 (R&D Systems, Minneapolis, MN, USA), GAPDH (Trevigen, Gaithersburg, MD, USA), and XIAP (Nordic Biosite, Täby, Sweden).

Techniques: Expressing, Derivative Assay, Labeling, Western Blot, Comparison